DNA Sensor for the Detection of Brucella spp. Based on Magnetic Nanoparticle Markers.

ABSTRACT: Due to the limitations of conventional Brucella detection methods, including safety concerns, long incubation times, and limited specificity, the development of a rapid, selective, and accurate technique for the early detection of Brucella in livestock animals is crucial to prevent the spread of the associated disease. In the present study, we introduce a magnetic nanoparticle marker-based biosensor using frequency mixing magnetic detection for point-of-care testing and quantification of Brucella DNA. Superparamagnetic nanoparticles were used as magnetically measured markers to selectively detect the target DNA hybridized with its complementary capture probes immobilized on a porous polyethylene filter. Experimental conditions like density and length of the probes, hyb

SUBMITTER: Abuawad A 

PROVIDER: S-EPMC10744106 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature