KuINins as a New Class of HIV-1 Inhibitors That Block Post-Integration DNA Repair.

ABSTRACT: Integration of HIV-1 genomic cDNA results in the formation of single-strand breaks in cellular DNA, which must be repaired for efficient viral replication. Post-integration DNA repair mainly depends on the formation of the HIV-1 integrase complex with the Ku70 protein, which promotes DNA-PK assembly at sites of integration and its activation. Here, we have developed a first-class inhibitor of the integrase-Ku70 complex formation that inhibits HIV-1 replication in cell culture by acting at the stage of post-integration DNA repair. This inhibitor, named s17, does not affect the main cellular function of Ku70, namely its participation in the repair of double-strand DNA breaks through the non-homologous end-joining pathway. Using a molecular dynamics approach, we have constructed a model for t

SUBMITTER: Anisenko A 

PROVIDER: S-EPMC10744174 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature