Effect of Bioactive Glasses and Basic Fibroblast Growth Factor on Dental Pulp Cells.

ABSTRACT: Ideal regeneration of hard tissue and dental pulp has been reported with the use of a combination of bioactive glass and basic fibroblast growth factor (bFGF). However, no previous study has investigated the molecular mechanisms underlying the processes induced by this combination in dental pulp cells. This study aimed to examine the cellular phenotype and transcriptional changes induced by the combination of bioactive glass solution (BG) and bFGF in dental pulp cells using phase-contrast microscopy, a cell counting kit-8 assay, alkaline phosphatase staining, and RNA sequence analysis. bFGF induced elongation of the cell process and increased the number of cells. Whereas BG did not increase ALP activity, it induced extracellular matrix-related genes in the dental pulp. In addition, the com

SUBMITTER: Washio A 

PROVIDER: S-EPMC10744375 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature