Oxidation Products of Tryptophan and Proline in Adipokinetic Hormones-Artifacts or Post-Translational Modifications?

ABSTRACT:

Background

Adipokinetic hormones (AKHs) regulate important physiological processes in insects. AKHs are short peptides with blocked termini and Trp in position 8. Often, proline occupies position 6. Few post-translational modifications have been found, including hydroxyproline ([Hyp⁶]) and kynurenine. Our recent data suggest that the Hyp- and Kyn-containing AKHs occur more often than originally thought and we here investigate if they are natural or artifactual.

Methods

From crude extracts of the corpora cardiaca (CC) of various insect species, AKHs were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-MS). Synthetic [Hyp⁶]-AKHs were tested in an in vivo metabolic assay. Freshly dissected Periplaneta americana

SUBMITTER: Konig S 

PROVIDER: S-EPMC10744910 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature