Microfluidic Diffusion Sizing Applied to the Study of Natural Products and Extracts That Modulate the SARS-CoV-2 Spike RBD/ACE2 Interaction.

ABSTRACT: The interaction between SARS-CoV-2 spike RBD and ACE2 proteins is a crucial step for host cell infection by the virus. Without it, the entire virion entrance mechanism is compromised. The aim of this study was to evaluate the capacity of various natural product classes, including flavonoids, anthraquinones, saponins, ivermectin, chloroquine, and erythromycin, to modulate this interaction. To accomplish this, we applied a recently developed a microfluidic diffusional sizing (MDS) technique that allows us to probe protein-protein interactions via measurements of the hydrodynamic radius (R_h) and dissociation constant (K_D); the evolution of R_h is monitored in the presence of increasing concentrations of the partner protein (ACE2); and the K_D is deter

SUBMITTER: Fauquet J 

PROVIDER: S-EPMC10745392 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature