Analysis of Softwood Lignans by Comprehensive Two-Dimensional Liquid Chromatography.

ABSTRACT: Lignans constitute a large group of phenolic plant secondary metabolites possessing high bioactivity. Their accurate determination in plant extracts with a complex chemical composition is challenging and requires advanced separation techniques. In the present study, a new approach to the determination of lignans in coniferous knotwood extracts as the promising industrial-scale source of such compounds based on comprehensive two-dimensional liquid chromatography separation and UV spectrophotometric detection is proposed. First and second-dimension column screening showed that the best results can be obtained using a combination of non-polar and polar hydroxy group embedded octadecyl stationary phases with moderate (~40%) "orthogonality". The optimization of LC × LC separation conditions all

SUBMITTER: Falev DI 

PROVIDER: S-EPMC10745517 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature