Quantitative Image Analysis of Axonal Morphology in In Vivo Model.

ABSTRACT: Quantifying axonal branching is crucial for understanding neural circuit function, developmental and regeneration processes and disease mechanisms. Factors that regulate patterns of axonal arborization and tune neuronal circuits are investigated for their implication in various disorders in brain connectivity. The lack of a reliable and user-friendly method makes the quantitative analysis of axon morphology difficult. Specifically, methods to visualize and quantify the complex axon arborization are challenging to implement and apply practically. Our study was aimed at developing a robust but simple method of quantification that used ImageJ 2D analysis and compared it with Imaris visualization and analysis of 3D images. We used zebrafish fluorescent transgenic lines to perform in vivo imagi

SUBMITTER: Nemoz-Billet L 

PROVIDER: S-EPMC10745806 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature