Dry Stamping Coral Powder: An Effective Method for Isolating Coral Symbiotic Actinobacteria.

ABSTRACT: Actinobacteria are important sources of antibiotics and have been found repeatedly in coral core microbiomes, suggesting this bacterial group plays important functional roles tied to coral survival. However, to unravel coral-actinobacteria ecological interactions and discover new antibiotics, the complex challenges that arise when isolating symbiotic actinobacteria must be overcome. Moreover, by isolating unknown actinobacteria from corals, novel biotechnological applications may be discovered. In this study, we compared actinobacteria recovery from coral samples between two widely known methods for isolating actinobacteria: dry stamping and heat shock. We found that dry stamping was at least three times better than heat shock. The assembly of isolated strains by dry stamping was unique fo

SUBMITTER: Becerril-Espinosa A 

PROVIDER: S-EPMC10745815 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature