The Restriction-Modification Systems of Clostridium carboxidivorans P7.

ABSTRACT: Clostridium carboxidivorans P7 (DSM 15243) is a bacterium that converts syngas (a mixture of CO, H₂, and CO₂) into hexanol. An optimized and scaled-up industrial process could therefore provide a renewable source of fuels and chemicals while consuming industry waste gases. However, the genetic engineering of this bacterium is hindered by its multiple restriction-modification (RM) systems: the genome of C. carboxidivorans encodes at least ten restriction enzymes and eight methyltransferases (MTases). To gain insight into the complex RM systems of C. carboxidivorans, we analyzed genomic methylation patterns using single-molecule real-time (SMRT) sequencing and bisulfite sequencing. We identified six methylated sequence motifs. To match the methylati

SUBMITTER: Kottenhahn P 

PROVIDER: S-EPMC10745947 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature