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Acid-Modulated Peptide Synthesis for Application on Oxide Biosensor Interfaces.

ABSTRACT: In this paper we report an acid-modulated strategy for novel peptide microarray production on biosensor interfaces. We initially selected a controlled pore glass (CPG) as a support for solid-phase peptide synthesis (SPPS) to implement a chemistry that can be performed at the interface of multiple field effect transistor (FET) sensors, eventually to generate label-free peptide microarrays for protein screening. Our chemistry uses a temporary protection of the N-terminal amino function of each amino acid building block with a tert-butyloxycarbonyl (Boc) group that can be removed after each SPPS cycle, in combination with semi-permanent protection of the side chains of trifunctional amino acid residues. Such a protection scheme with a well-proven record of application in conventional, batchwise SPPS has been fine-tuned for optimal performance on CPG and, from there, translated to SPR chips that allow layer-by-layer monitoring of amino acid coupling. Our results validate this acid-modulated synthesis as a feasible approach for producing peptides in high yields and purity on flat glass surfaces, such as those in bio-FETs.

SUBMITTER: Cristobal-Lecina E

PROVIDER: S-EPMC10746054 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Acid-Modulated Peptide Synthesis for Application on Oxide Biosensor Interfaces.

Cristóbal-Lecina Edgar E El-Maiss Janwa J Figueras Eduard E Singh Aruna Chandra AC Krishnamoorthy Sivashankar S Østerbye Thomas T Pascual García César C Andreu David D

Nanomaterials (Basel, Switzerland) 20231206 24

In this paper we report an acid-modulated strategy for novel peptide microarray production on biosensor interfaces. We initially selected a controlled pore glass (CPG) as a support for solid-phase peptide synthesis (SPPS) to implement a chemistry that can be performed at the interface of multiple field effect transistor (FET) sensors, eventually to generate label-free peptide microarrays for protein screening. Our chemistry uses a temporary protection of the N-terminal amino function of each ami ...[more]

PMID: 38132988

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Project description:Conducting polymers (CPs), especially polyaniline (PANI) based hybrid materials have emerged as very interesting materials for the adsorption of heavy metals and dyes from an aqueous environment due to their electrical transport properties, fascinating doping/de-doping chemistry and porous surface texture. Acid Blue 40 (AB40) is one of the common dyes present in the industrial effluents. We have performed a comparative study on the removal of AB40 from water through the application of PANI, magnetic oxide (Fe3O4) and their composites. Prior to this study, PANI and its composites with magnetic oxide were synthesized through our previously reported chemical oxidative synthesis route. The adsorption of AB40 on the synthesized materials was investigated with UV-Vis spectroscopy and resulting data were analyzed by fitting into Tempkin, Freundlich, Dubinin-Radushkevich (D-R) and Langmuir isotherm models. The Freundlich isotherm model fits more closely to the adsorptions data with R2 values of 0.933, 0.971 and 0.941 for Fe3O4, PANI and composites, respectively. The maximum adsorption capacity of Fe3O4, PANI and composites was, respectively, 130.5, 264.9 and 216.9 mg g-1. Comparatively good adsorption capability of PANI in the present case is attributed to electrostatic interactions and a greater number of H-bonding. Effect of pH of solution, temperature, initial concentration of AB40, contact time, ionic strength and dose of adsorbent were also investigated. Adsorption followed pseudo-second-order kinetics. The activation energy of adsorption of AB40 on Fe3O4, PANI and composites were 30.12, 22.09 and 26.13 kJmol-1 respectively. Enthalpy change, entropy change and Gibbs free energy changes are -6.077, -0.026 and -11.93 kJ mol-1 for adsorption of AB40 on Fe3O4. These values are -8.993, -0.032 and -19.87 kJ mol-1 for PANI and -10.62, -0.054 and -19.75 kJ mol-1 for adsorption of AB40 on PANI/Fe3O4 composites. The negative sign of entropy, enthalpy and Gibbs free energy changes indicate spontaneous and exothermic nature of adsorption.

Dataset Information

Acid-Modulated Peptide Synthesis for Application on Oxide Biosensor Interfaces.

Publications

Acid-Modulated Peptide Synthesis for Application on Oxide Biosensor Interfaces.

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