Ribosome profiling reveals the fine-tuned response of <i>Escherichia coli</i> to mild and severe acid stress.

ABSTRACT:

Importance

Bacteria react very differently to survive in acidic environments, such as the human gastrointestinal tract. Escherichia coli is one of the extremely acid-resistant bacteria and has a variety of acid-defense mechanisms. Here, we provide the first genome-wide overview of the adaptations of E. coli K-12 to mild and severe acid stress at both the transcriptional and translational levels. Using ribosome profiling and RNA sequencing, we uncover novel adaptations to different degrees of acidity, including previously hidden stress-induced small proteins and novel key transcription factors for acid defense, and report mRNAs with pH-dependent differential translation efficiency. In addition, we distinguish between acid-specific adaptations and general stress respons

SUBMITTER: Schumacher K 

PROVIDER: S-EPMC10746267 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature