Filamentous actin destabilization by H₂O₂ favors DnmA aggregation, with crucial roles of cysteines 450 and 776 in mitochondrial and peroxisomal division in Aspergillus nidulans.

ABSTRACT:

Importance

Mitochondria constitute major sources of H₂O₂ and other reactive oxygen species in eukaryotic cells. The division of these organelles is crucial for multiple processes in cell biology and relies on highly regulated mechano-GTPases that are oligomerization dependent and belong to the dynamin-related protein family, like A. nidulans DnmA. Our previous work demonstrated that H₂O₂ induces mitochondrial constriction, division, and remodeling of the outer membrane. Here, we show that H₂O₂ also induces a DnmA aggregation consistent with higher-order oligomerization and its recruitment to mitochondria. The study of this response uncovered that H₂O₂ induces the depolymerization and reor

SUBMITTER: Garrido-Bazan V 

PROVIDER: S-EPMC10746283 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature