Nitrogen fixation and transcriptome of a new diazotrophic Geomonas from paddy soils.

ABSTRACT: Nitrogen gas (N₂) fixation driven by diazotrophs is a crucial process for supplying nitrogen to paddy soil ecosystems. The genus Geomonas has been considered to be an important potential diazotroph in paddy soils, but direct experimental evidence of the nitrogen-fixing ability of Geomonas in pure culture is still lacking. Hence, we aimed to demonstrate this nitrogen-fixing capability and shed light on how this process was regulated in response to ammonium (NH₄ ⁺) in Geomonas. In this study, we determined that a key nitrogenase gene (nifH) was present in 50 isolates from paddy soils. Members of Geomonas contained the minimum nitrogen fixation gene cluster (nifBHDKEN) based on genomic analysis, implying Geomonas

SUBMITTER: Liu G-H 

PROVIDER: S-EPMC10746287 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature