Small transcriptional differences among cell clones lead to distinct NF-κB dynamics.

ABSTRACT: Transcription factor dynamics is fundamental to determine the activation of accurate transcriptional programs and yet is heterogeneous at a single-cell level, even within homogeneous populations. We asked how such heterogeneity emerges for the nuclear factor κB (NF-κB). We found that clonal populations of immortalized fibroblasts derived from a single mouse embryo display robustly distinct NF-κB dynamics upon tumor necrosis factor ɑ (TNF-ɑ) stimulation including persistent, oscillatory, and weak activation, giving rise to differences in the transcription of its targets. By combining transcriptomics and simulations we show how less than two-fold differences in the expression levels of genes coding for key proteins of the signaling cascade and feedback system are predictive of the difference

SUBMITTER: Kizilirmak C 

PROVIDER: S-EPMC10746373 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature