Baicalin attenuates pulmonary vascular remodeling by inhibiting calpain-1 mediated endothelial-to-mesenchymal transition.

ABSTRACT:

Background

Previous studies have demonstrated the beneficial effect of baicalin on pulmonary arterial hypertension (PAH), but the mechanism is unclear.

Aim

The aim of the present study was to evaluate the effect of baicalin on pulmonary vascular remodeling (PVR) with a focus on calpain-1-mediated endothelial-to-mesenchymal transition (EndMT).

Methods

PAH was induced by intraperitoneal injection of monocrotaline (MCT) in rats and hypoxia in calpain-1 gene knockout (Capn1^-/-) and wild-type C57BL/6 mice. An in vitro PVR model was established in PASMCs and HPAECs.

Results

The data showed that baicalin treatment and calpain-1 inhibition alleviated MCT and hypoxia-induced increases in right ventricular systolic pressure (RVSP), prevented right ventricle hy

SUBMITTER: Jiang HX 

PROVIDER: S-EPMC10746466 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature