Long-term super-resolution inner mitochondrial membrane imaging with a lipid probe.

ABSTRACT: The inner mitochondrial membrane (IMM) generates power to drive cell function, and its dynamics control mitochondrial health and cellular homeostasis. Here, we describe the cell-permeant, lipid-like small molecule MAO-N₃ and use it to assemble high-density environmentally sensitive (HIDE) probes that selectively label and image the IMM in live cells and multiple cell states. MAO-N₃ pairs with strain-promoted azide-alkyne click chemistry-reactive fluorophores to support HIDE imaging using confocal, structured illumination, single-molecule localization and stimulated emission depletion microscopy, all with significantly improved resistance to photobleaching. These probes generate images with excellent spatial and temporal resolution, require no genetic manipulations, ar

SUBMITTER: Zheng S 

PROVIDER: S-EPMC10746544 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature