Contrasting effects of whole-body and hepatocyte-specific deletion of the RNA polymerase III repressor Maf1 in the mouse.

ABSTRACT: MAF1 is a nutrient-sensitive, TORC1-regulated repressor of RNA polymerase III (Pol III). MAF1 downregulation leads to increased lipogenesis in Drosophila melanogaster, Caenorhabditis elegans, and mice. However, Maf1 ^-/- mice are lean as increased lipogenesis is counterbalanced by futile pre-tRNA synthesis and degradation, resulting in increased energy expenditure. We compared Chow-fed Maf1 ^-/- mice with Chow- or High Fat (HF)-fed Maf1 ^hep-/- mice that lack MAF1 specifically in hepatocytes. Unlike Maf1 ^-/- mice, Maf1 ^hep-/- mice become heavier and fattier than control mice with old age and much earlier under a HF diet. Liver ChIPseq, RNAseq and proteomic

SUBMITTER: Willemin G 

PROVIDER: S-EPMC10746880 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature