Identification of Adenovirus E1B-55K Interaction Partners through a Common Binding Motif.

ABSTRACT: The adenovirus C5 E1B-55K protein is crucial for viral replication and is expressed early during infection. It can interact with E4orf6 to form a complex that functions as a ubiquitin E3 ligase. This complex targets specific cellular proteins and marks them for ubiquitination and, predominantly, subsequent proteasomal degradation. E1B-55K interacts with various proteins, with p53 being the most extensively studied, although identifying binding sites has been challenging. To explain the diverse range of proteins associated with E1B-55K, we hypothesized that other binding partners might recognize the simple p53 binding motif (xWxxxPx). In silico analyses showed that many known E1B-55K binding proteins possess this amino acid sequence; therefore, we investigated whether other xWxxxPx-containi

SUBMITTER: Chalabi Hagkarim N 

PROVIDER: S-EPMC10747525 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature