A Multiplex PCR System of Novel Microsatellite Loci for Population Genetic Application in Walnuts.

ABSTRACT: Multiplex polymerase chain reaction (PCR) of microsatellite loci allows for simultaneous amplification of two or more pairs of primers in a single PCR reaction; hence, it is cost and time effective. However, very few attempts have been reported in non-model species. In this study, by combining a genome-based de novo development and cross-species application approach, a multiplex PCR system comprising 5 PCR reactions of 33 microsatellites consisting of 26 novel genomic and 7 literature-sourced loci was tested for polymorphisms, cross-species transferability, and the ability to assess genetic diversity and population structure of three walnut species (Juglans spp.). We found that the genome-based approach is more efficient than other methods. An allelic ladder was developed for each l

SUBMITTER: Xiahou ZY 

PROVIDER: S-EPMC10747719 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature