Proteomic Approach during the Induction of Somatic Embryogenesis in Coffea canephora.

ABSTRACT: Plant growth regulators (PGR) are essential for somatic embryogenesis (SE) in different species, and Coffea canephora is no exception. In our study model, previously, we have been able to elucidate the participation of various genes involved in SE by using different strategies; however, until now, we have not used a proteomic approach. This research seeks to contribute to understanding the primary cellular pathways involved in developing SE in C. canephora. The process of our model consists of two stages: (1) preconditioning in MS medium with auxin (NAA) and cytokinin (KIN), and (2) induction in Yasuda liquid medium added with cytokinin (BA). Therefore, in this study, we analyzed different days of the SE induction process using shotgun label-free proteomics. An amount of 1630

SUBMITTER: Quintana-Escobar AO 

PROVIDER: S-EPMC10748034 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature