Developing a Toolbox of Antibodies Validated for Array Tomography-Based Imaging of Brain Synapses.

ABSTRACT: Antibody (Ab)-based imaging techniques rely on reagents whose performance may be application specific. Because commercial antibodies are validated for only a few purposes, users interested in other applications may have to perform extensive in-house antibody testing. Here, we present a novel application-specific proxy screening step to efficiently identify candidate antibodies for array tomography (AT), a serial section volume microscopy technique for high-dimensional quantitative analysis of the cellular proteome. To identify antibodies suitable for AT-based analysis of synapses in mammalian brain, we introduce a heterologous cell-based assay that simulates characteristic features of AT, such as chemical fixation and resin embedding that are likely to influence antibody binding. The assay

SUBMITTER: Micheva KD 

PROVIDER: S-EPMC10748464 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature