Enabling genome editing in tropical maize lines through an improved, morphogenic regulator-assisted transformation protocol.

ABSTRACT: The recalcitrance exhibited by many maize (Zea mays) genotypes to traditional genetic transformation protocols poses a significant challenge to the large-scale application of genome editing (GE) in this major crop species. Although a few maize genotypes are widely used for genetic transformation, they prove unsuitable for agronomic tests in field trials or commercial applications. This challenge is exacerbated by the predominance of transformable maize lines adapted to temperate geographies, despite a considerable proportion of maize production occurring in the tropics. Ectopic expression of morphogenic regulators (MRs) stands out as a promising approach to overcome low efficiency and genotype dependency, aiming to achieve 'universal' transformation and GE capabilities in maize. Her

SUBMITTER: Hernandes-Lopes J 

PROVIDER: S-EPMC10748596 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature