Prior viral infection primes cross-reactive CD8+ T cells that respond to mouse heart allografts.

ABSTRACT:

Introduction

Significant evidence suggests a connection between transplant rejection and the presence of high levels of pre-existing memory T cells. Viral infection can elicit viral-specific memory T cells that cross-react with allo-MHC capable of driving allograft rejection in mice. Despite these advances, and despite their critical role in transplant rejection, a systematic study of allo-reactive memory T cells, their specificities, and the role of cross-reactivity with viral antigens has not been performed.

Methods

Here, we established a model to identify, isolate, and characterize cross-reactive T cells using Nur77 reporter mice (C57BL/6 background), which transiently express GFP exclusively upon TCR engagement. We infected Nur77 mice with lymphocytic choriomeningitis vi

SUBMITTER: Khorki ME 

PROVIDER: S-EPMC10748599 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature