Cancer susceptibility 18 positively regulates NUAK Family Kinase 1 expression to promote migration and invasion via sponging of miR-5586-5p in cervical cancer cells.

ABSTRACT:

Introduction

Cervical squamous cell carcinoma (CESC) is the most common gynecological malignancy worldwide. Although the cancer susceptibility 18 (CASC18) gene was involved in the regulation of cancer biology, its specific role in CESC is not well characterized.

Methods

CASC18-related axis was predicted by bioinformatic analyses, and the competing endogenous RNA (ceRNA) interaction was further validated using quantitative real-time PCR, western blotting, RNA pulldown, and luciferase reporter assays. Transwell and wound healing assays were performed to verify the effect of CASC18 on SiHa and HeLa cell motility.

Results

We found that CASC18 was upregulated in CESC tissues. Moreover, interference with CASC18 attenuated NUAK1-medi

SUBMITTER: Wang J 

PROVIDER: S-EPMC10748670 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature