Biophysical characterization of the Plasmodium falciparum circumsporozoite protein's N-terminal domain.

ABSTRACT: The circumsporozoite protein (CSP) is the main surface antigen of the Plasmodium sporozoite (SPZ) and forms the basis of the currently only licensed anti-malarial vaccine (RTS,S/AS01). CSP uniformly coats the SPZ and plays a pivotal role in its immunobiology, in both the insect and the vertebrate hosts. Although CSP's N-terminal domain (CSP_N ) has been reported to play an important role in multiple CSP functions, a thorough biophysical and structural characterization of CSP_N is currently lacking. Here, we present an alternative method for the recombinant production and purification of CSP_N from Plasmodium falciparum (PfCSP_N ), which provides pure, high-quality protein preparations with high yields. Through an interdisciplinary approach combining

SUBMITTER: Geens R 

PROVIDER: S-EPMC10749493 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature