Conditioned medium from human dental pulp stem cells treats spinal cord injury by inhibiting microglial pyroptosis.

ABSTRACT: Human dental pulp stem cell transplantation has been shown to be an effective therapeutic strategy for spinal cord injury. However, whether the human dental pulp stem cell secretome can contribute to functional recovery after spinal cord injury remains unclear. In the present study, we established a rat model of spinal cord injury based on impact injury from a dropped weight and then intraperitoneally injected the rats with conditioned medium from human dental pulp stem cells. We found that the conditioned medium effectively promoted the recovery of sensory and motor functions in rats with spinal cord injury, decreased expression of the microglial pyroptosis markers NLRP3, GSDMD, caspase-1, and interleukin-1β, promoted axonal and myelin regeneration, and inhibited the formation of glial sc

SUBMITTER: Liu T 

PROVIDER: S-EPMC10749599 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature