MtFRC: depth-dependent resolution quantification of image features in 3D fluorescence microscopy.

ABSTRACT:

Motivation

Quantifying lateral resolution as a function of depth is important in the design of 3D microscopy experiments. However, for many specimens, resolution is non-uniform within the same optical plane because of factors such as tissue variability and differential light scattering. This precludes application of a simple resolution metric to the image as a whole. In such cases, it can be desirable to analyse resolution only within specific, well-defined features.

Results

An algorithm and software are presented to characterize resolution as a function of depth in features of arbitrary shape in 3D samples. The tool can be used to achieve an objective comparison between different preparation methods, imaging parameters, and optical systems. It can also inform the design of

SUBMITTER: Wright N 

PROVIDER: S-EPMC10749749 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature