Hostile: accurate decontamination of microbial host sequences.

ABSTRACT:

Motivation

Microbial sequences generated from clinical samples are often contaminated with human host sequences that must be removed for ethical and legal reasons. Care must be taken to excise host sequences without inadvertently removing target microbial sequences to the detriment of downstream analyses such as variant calling and de novo assembly.

Results

To facilitate accurate host decontamination of both short and long sequencing reads, we developed Hostile, a tool capable of accurate host read removal using a laptop. We demonstrate that our approach removes at least 99.6% of real human reads and retains at least 99.989% of simulated bacterial reads. Using Hostile with a masked reference genome further increases bacterial read retention (≥99.997%) with negligible (≤0.001

SUBMITTER: Constantinides B 

PROVIDER: S-EPMC10749771 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature