Selection and validation of genes related to oxidative stress production and clearance in macrophages infected with Mycobacterium tuberculosis.

ABSTRACT:

Background

In the fight against tuberculosis, besides chemotherapy, the regulation of oxidative stress (OS) has also aroused people's interest in host-oriented therapy. However, there is limited research on the genes involved in reactive oxygen species (ROS) production and clearance in macrophages infected with Mycobacterium tuberculosis (MTB). This study analyzes and explores this to provide a basis for exploring new targets for antituberculosis treatments.

Methods

We established a macrophage model infected with MTB, counted intracellular bacteria, and determined the ROS produced using flow cytometry. We conducted ribonucleic acid sequencing, screened differentially expressed genes through transcriptomic methods, and validated the expression of them through reverse t

SUBMITTER: Su R 

PROVIDER: S-EPMC10749926 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature