Enrichment of sweat-derived extracellular vesicles of human and bacterial origin for biomarker identification.

ABSTRACT: Sweat contains biomarkers for real-time non-invasive health monitoring, but only a few relevant analytes are currently used in clinical practice. In the present study, we investigated whether sweat-derived extracellular vesicles (EVs) can be used as a source of potential protein biomarkers of human and bacterial origin. Methods: By using ExoView platform, electron microscopy, nanoparticle tracking analysis and Western blotting we characterized EVs in the sweat of eight volunteers performing rigorous exercise. We compared the presence of EV markers as well as general protein composition of total sweat, EV-enriched sweat and sweat samples collected in alginate skin patches. Results: We identified 1209 unique human proteins in EV-enriched sweat, of which approximately 20% were p

SUBMITTER: Zhyvolozhnyi A 

PROVIDER: S-EPMC10750121 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature