A natural large chromosomal inversion in Lactococcus lactis is mediated by homologous recombination between two insertion sequences.

ABSTRACT: Comparative analysis of chromosomal macrorestriction polymorphism of the two closely related Lactococcus lactis subsp. cremoris strains MG1363 and NCDO763 revealed the presence of a large inversion covering half of the genome. To determine what kind of genetic element could be implicated in this rearrangement, the two inversion junctions of MG1363 and NCDO763 chromosomes were cloned and characterized. Nucleotide sequence analysis showed the presence of one copy of the lactococcal IS905 element in each junction. Each copy of this element contained the same nucleotide mutation that inactivates the putative transposase. Comparison of the sequences surrounding the insertion sequence demonstrated that the large inversion arose from a single-step homologous recombination event between the two de

SUBMITTER: Daveran-Mingot ML 

PROVIDER: S-EPMC107507 | biostudies-literature | 1998 Sep

REPOSITORIES: biostudies-literature