Differential effects of Western diet and traumatic muscle injury on skeletal muscle metabolic regulation in male and female mice.

ABSTRACT:

Background

This study was designed to develop an understanding of the pathophysiology of traumatic muscle injury in the context of Western diet (WD; high fat and high sugar) and obesity. The objective was to interrogate the combination of WD and injury on skeletal muscle mass and contractile and metabolic function.

Methods

Male and female C57BL/6J mice were randomized into four groups based on a two-factor study design: (1) injury (uninjured vs. volumetric muscle loss [VML]) and (2) diet (WD vs. normal chow [NC]). Electrophysiology was used to test muscle strength and metabolic function in cohorts of uninjured + NC, uninjured + WD, VML + NC and VML + WD at 8 weeks of intervention.

Results

VML-injured male and female mice both exhibited decrements in muscle mass (-17%,

SUBMITTER: Heo J 

PROVIDER: S-EPMC10751418 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature