Lactate promotes myogenesis via activating H3K9 lactylation-dependent up-regulation of Neu2 expression.

ABSTRACT:

Background

Lactate, a glycolytic metabolite mainly produced in muscles, has been suggested to regulate myoblast differentiation, although the underlying mechanism remains elusive. Recently, lactate-mediated histone lactylation is identified as a novel epigenetic modification that promotes gene transcription.

Methods

We used mouse C2C12 cell line and 2-month-old male mice as in vitro and in vivo models, respectively. These models were treated with lactate to explore the biological function and latent mechanism of lactate-derived histone lactylation on myogenic differentiation by quantitative real-time PCR, western blotting, immunofluorescence staining, chromatin immunoprecipitation, cleavage under targets and tagmentation assay and RNA sequencing.

Results

Using immunof

SUBMITTER: Dai W 

PROVIDER: S-EPMC10751423 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature