Ontology highlight
ABSTRACT:
SUBMITTER: Peterson BG
PROVIDER: S-EPMC10751623 | biostudies-literature | 2023 Nov
REPOSITORIES: biostudies-literature
Peterson Brian G BG Hwang Jiwon J Russ Jennifer E JE Schroeder Jeremy W JW Freddolino P Lydia PL Baldridge Ryan D RD
Cell reports 20231118 11
Misfolded endoplasmic reticulum (ER) proteins are degraded through a process called ER-associated degradation (ERAD). Soluble, lumenal ERAD targets are recognized, retrotranslocated across the ER membrane, ubiquitinated, extracted from the membrane, and degraded by the proteasome using an ERAD pathway containing a ubiquitin ligase called Hrd1. To determine how Hrd1 mediates these processes, we developed a deep mutational scanning approach to identify residues involved in Hrd1 function, including ...[more]