Two genes encoded by mulberry crinkle leaf virus (MCLV): The V4 gene enhances viral replication, and the V5 gene is needed for MCLV infection in Nicotiana benthamiana.

ABSTRACT: Mulberry crinkle leaf virus (MCLV) is a member of the genus Mulcrilevirus, family Geminiviridae. The expression and functions of the V4 and V5 genes encoded by the MCLV genome remain unknown. Here, we confirmed the expression of V4 and V5 by analyzing the V4 and V5 mRNAs and the promoter activity of individual ORFs upstream sequences. The functions of V4 and V5 were investigated by constructing Agrobacterium-mediated infectious clones of wild-type MCLV variant П (MCLV vII), MCLV^wt and MCLV vП mutants, such as MCLV^mV4 (start codon of V4 ORF mutated), MCLV^dV4 (5'-end partial deletion of V4 ORF sequence) and MCLV^mV5 (V5 ORF start codon mutated). Although MCLV^wt, MCLV^mV4, and MCLV^dV4 could infect natural host mulberry

SUBMITTER: Han TT 

PROVIDER: S-EPMC10751690 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature