PET Imaging of Innate Immune Activation Using ¹¹C Radiotracers Targeting GPR84.

ABSTRACT: Chronic innate immune activation is a key hallmark of many neurological diseases and is known to result in the upregulation of GPR84 in myeloid cells (macrophages, microglia, and monocytes). As such, GPR84 can potentially serve as a sensor of proinflammatory innate immune responses. To assess the utility of GPR84 as an imaging biomarker, we synthesized ¹¹C-MGX-10S and ¹¹C-MGX-11Svia carbon-11 alkylation for use as positron emission tomography (PET) tracers targeting this receptor. In vitro experiments demonstrated significantly higher binding of both radiotracers to hGPR84-HEK293 cells than that of parental control HEK293 cells. Co-incubation with the GPR84 antagonist GLPG1205 reduced the binding of both radiotracers by >90%,

SUBMITTER: Kalita M 

PROVIDER: S-EPMC10751761 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature