Control points for design of taxonomic composition in synthetic human gut communities.

ABSTRACT: Microbial communities offer vast potential across numerous sectors but remain challenging to systematically control. We develop a two-stage approach to guide the taxonomic composition of synthetic microbiomes by precisely manipulating media components and initial species abundances. By combining high-throughput experiments and computational modeling, we demonstrate the ability to predict and design the diversity of a 10-member synthetic human gut community. We reveal that critical environmental factors governing monoculture growth can be leveraged to steer microbial communities to desired states. Furthermore, systematically varied initial abundances drive variation in community assembly and enable inference of pairwise inter-species interactions via a dynamic ecological model. These intera

SUBMITTER: Connors BM 

PROVIDER: S-EPMC10752370 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature