RNA-seq characterization of histamine-releasing mast cells as potential therapeutic target of osteoarthritis.

ABSTRACT:

Objective

Mast cells in the osteoarthritis (OA) synovium correlate with disease severity. This study aimed to further elucidate the role of mast cells in OA by RNA-Seq analysis and pharmacological blockade of the activity of histamine, a key mast cell mediator, in murine OA.

Methods

We examined OA synovial tissues and fluids by flow cytometry, immunostaining, single-cell and bulk RNA-Seq, qPCR, and ELISA. Cetirizine, a histamine H1 receptor (H₁R) antagonist, was used to treat the destabilization of the medial meniscus (DMM) mouse model of OA.

Results

Flow cytometry and immunohistology analysis of OA synovial cells revealed KIT⁺ FcεRI⁺ and TPSAB1⁺ mast cells. Single-cell RNA-Seq of OA synovial cells identified the expression

SUBMITTER: Zhao X 

PROVIDER: S-EPMC10752578 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature