Single-cell RNA sequencing analysis reveals the heterogeneity of IL-10 producing regulatory B cells in lupus-prone mice.

ABSTRACT:

Introduction

B cells can have both pathogenic and protective roles in autoimmune diseases, including systemic lupus erythematosus (SLE). Deficiencies in the number or immunosuppressive function of IL-10 producing regulatory B cells (Bregs) can cause exacerbated autoimmune inflammation. However, the exact role of Bregs in lupus pathogenesis has not been elucidated.

Methods

We carried out gene expression analysis by scRNA-seq to characterize differences in splenic Breg subsets and molecular profiles through stages of disease progression in lupus-prone mice. Transcriptome-based changes in Bregs from mice with active disease were confirmed by phenotypic analysis.

Results

We found that a loss of marginal zone (MZ) lineage Bregs, an increase in plasmablast/plasma cell (PB-P

SUBMITTER: Daamen AR 

PROVIDER: S-EPMC10752970 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature