Evaluation of whole-genome enrichment and sequencing of T. pallidum from FFPE samples after 75 years.

ABSTRACT: The recent developments in genomic sequencing have permitted the publication of many new complete genome sequences of Treponema pallidum pallidum, the bacterium responsible for syphilis, which has led to a new understanding of its phylogeny and diversity. However, few archived samples are available, because of the degradability of the bacterium and the difficulties in preservation. We present a complete genome obtained from a Formalin-Fixed Paraffin-Embedded (FFPE) organ sample from 1947, kept at the Strasbourg Faculty of Medicine. This is the preliminary, proof-of concept study of this collection/biobank of more than 1.5 million FFPE samples and the evaluation of the feasibility of genomic analyses. We demonstrate here that even degraded DNA from fragile bacteria can be recovered f

SUBMITTER: Zvenigorosky V 

PROVIDER: S-EPMC10753063 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature