Real-time monitoring of the amyloid β_1-42 monomer-to-oligomer channel transition using a lipid bilayer system.

ABSTRACT: This study describes the observation of the transformation of monomeric amyloid β_1-42 (Aβ42) into oligomers in a lipid membrane utilizing a lipid bilayer system for electrophysiological measurement. The relevance of oligomers and protofibrils in Alzheimer's disease (AD) is underscored given their significant neurotoxicity. By closely monitoring the shift of Aβ42 from its monomeric state to forming oligomeric channels in phospholipid membranes, we noted that this transformation transpired within a 2-h frame. We manipulated the lipid membrane's constitution with components such as glycerophospholipid, porcine brain total lipid extract, sphingomyelin (SM), and cholesterol (Chol.) to effectively imitate nerve cell membranes. Interesting findings showcased Chol.'s ability to foster s

SUBMITTER: Numaguchi Y 

PROVIDER: S-EPMC10753159 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature