SpliceWiz: interactive analysis and visualization of alternative splicing in R.

ABSTRACT: Alternative splicing (AS) is a crucial mechanism for regulating gene expression and isoform diversity in eukaryotes. However, the analysis and visualization of AS events from RNA sequencing data remains challenging. Most tools require a certain level of computer literacy and the available means of visualizing AS events, such as coverage and sashimi plots, have limitations and can be misleading. To address these issues, we present SpliceWiz, an R package with an interactive Shiny interface that allows easy and efficient AS analysis and visualization at scale. A novel normalization algorithm is implemented to aggregate splicing levels within sample groups, thereby allowing group differences in splicing levels to be accurately visualized. The tool also offers downstream gene ontology enrichme

SUBMITTER: Wong ACH 

PROVIDER: S-EPMC10753292 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature