Activation of the 5-hydroxytryptamine 4 receptor ameliorates tight junction barrier dysfunction in the colon of type 1 diabetic mice.

ABSTRACT: Hyperglycemia drives dysfunction of the intestinal barrier. 5-Hydroxytryptaine 4 receptor (5-HT ₄R) agonists have been considered therapeutics for constipation in clnic. However, the roles of 5-HT ₄R activation in mucosa should be fully realized. Here, we investigate the effects of 5-HT ₄R activation on diabetes-induced disruption of the tight junction (TJ) barrier in the colon. Not surprisingly, the TJ barrier in diabetic mice with or without 5-HT ₄R is tremendously destroyed, as indicated by increased serum fluorescein isothiocyanate (FITC)-dextran and decreased transepithelial electrical resistance (TER). Simultaneously, decreased expressions of TJ proteins are shown in both wild-type (WT) and 5-HT ₄R knockout (KO) mice with diabete

SUBMITTER: Hu S 

PROVIDER: S-EPMC10753360 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature