VIGA: a one-stop tool for eukaryotic virus identification and genome assembly from next-generation-sequencing data.

ABSTRACT: Identification of viruses and further assembly of viral genomes from the next-generation-sequencing data are essential steps in virome studies. This study presented a one-stop tool named VIGA (available at https://github.com/viralInformatics/VIGA) for eukaryotic virus identification and genome assembly from NGS data. It was composed of four modules, namely, identification, taxonomic annotation, assembly and novel virus discovery, which integrated several third-party tools such as BLAST, Trinity, MetaCompass and RagTag. Evaluation on multiple simulated and real virome datasets showed that VIGA assembled more complete virus genomes than its competitors on both the metatranscriptomic and metagenomic data and performed well in assembling virus genomes at the strain level. Finally, VIGA was use

SUBMITTER: Fu P 

PROVIDER: S-EPMC10753531 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature