Precision measurements of relaxation rates of degenerate ¹H transitions in methyl groups of small proteins.

ABSTRACT: An NMR experiment is designed for accurate and robust measurement of transverse relaxation rates of degenerate ¹H transitions in selectively ¹³CH₃-labeled, deuterated small proteins. The measurement is based on the use of acute (<90°) angle ¹H radio-frequency pulses and relies on selection of the slow- and fast-relaxing components of methyl magnetization following the relaxation period in separate experiments. The R₂ decay series recorded with selection of the fast-relaxing components serves as a useful complement to the R₂ series acquired with selection of the slow-relaxing part, and permits the extension of the range of relative contributions of the fast- and slow-relaxing parts to apparent signal decay. The approach is ex

SUBMITTER: Tugarinov V 

PROVIDER: S-EPMC10753654 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature