Dose-response technique combined with stable isotope tracing for drug metabolite profiling by using high-resolution mass spectrometry.

ABSTRACT: Background: Mass spectrometry metabolomics-based data-processing approaches have been developed for drug metabolite profiling. However, existing approaches cannot be used to comprehensively identify drug metabolites with high efficacy. Methods: Herein, we propose a two-stage data-processing approach for effective and comprehensive drug metabolite identification. The approach combines dose-response experiments with stable isotope tracing (SIT). Rosiglitazone (ROS), commonly used to treat type 2 diabetes, was employed as a model drug. Results: In the first stage of data processing, 1,071 features exhibited a dose-response relationship among 22,597 features investigated. In the second stage, these 1,071 features were screened for isotope pairs, and 200 features with isoto

SUBMITTER: Lin IS 

PROVIDER: S-EPMC10753831 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature