Synthetic Biology Toolbox for Nitrogen-Fixing Soil Microbes.

ABSTRACT: The soil environment adjacent to plant roots, termed the rhizosphere, is home to a wide variety of microorganisms that can significantly affect the physiology of nearby plants. Microbes in the rhizosphere can provide nutrients, secrete signaling compounds, and inhibit pathogens. These processes could be manipulated with synthetic biology to enhance the agricultural performance of crops grown for food, energy, or environmental remediation, if methods can be implemented in these nonmodel microbes. A common first step for domesticating nonmodel organisms is the development of a set of genetic engineering tools, termed a synthetic biology toolbox. A toolbox comprises transformation protocols, replicating vectors, genome engineering (e.g., CRISPR/Cas9), constitutive and inducible promoter syste

SUBMITTER: Venkataraman M 

PROVIDER: S-EPMC10754042 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature