Sperm induce a secondary increase in ATP levels in mouse eggs that is independent of Ca2+ oscillations.

ABSTRACT: Egg activation at fertilization in mouse eggs is caused by a series of cytosolic Ca2+ oscillations that are associated with an increase in ATP concentrations driven by increased mitochondrial activity. We have investigated the role of Ca2+ oscillations in these changes in ATP at fertilization by measuring the dynamics of ATP and Ca2+ in mouse eggs. An initial ATP increase started with the first Ca2+ transient at fertilization and then a secondary increase in ATP occurred ∼1 h later and this preceded a small and temporary increase in the frequency of Ca2+ oscillations. Other stimuli that caused Ca2+ oscillations such as PLCz1 or thimerosal, caused smaller or slower changes in ATP that failed to show the distinct secondary rise. Sperm-induced Ca2+ oscillations in the egg also triggered chang

SUBMITTER: Ikie-Eshalomi C 

PROVIDER: S-EPMC10754276 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature