A pH-sensitive eosin-block copolymer delivers proteins intracellularly.
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ABSTRACT:
SUBMITTER: Reinhard S
PROVIDER: S-EPMC10754351 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
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A pH-sensitive eosin-block copolymer delivers proteins intracellularly.
Reinhard Sören S Han Hesong H Tuma Jan J Røise Joachim Justad JJ Li I-Che IC Li Jie J Lee Hye Young HY Murthy Niren N
Chemical communications (Cambridge, England) 20201028 91
There is great interest in developing strategies to deliver proteins into the cytoplasm of cells. We report here a PEG-poly-eosin block copolymer (PEG-pEosin) that can encapsulate proteins and release them in active form under mildly acidic conditions. A PEG-pEosin formulation composed of Cre and the endosomolytic protein LLO efficiently performed gene editing in cells and in the brains of mice after an intracranial injection. ...[more]
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