Relationship between antimicrobial peptides-induced cell membrane damage and bactericidal activity.

ABSTRACT: Most antimicrobial peptides (AMPs) act by killing bacterial cells. However, there is little information regarding the required interaction time between AMPs and bacterial cells to exert the bactericidal activity. One of the causes of the bactericidal activity is considered to be cell membrane damage, although little direct evidence is available. Here, we investigated the relationship between AMP-induced cell membrane damage in Escherichia coli and AMP-induced cell death at the single-cell level. Magainin 2, lactoferricin B, and PGLa were selected as the AMPs. First, we examined the interaction time (t) of AMPs with cells required to induce cell death using the single-cell analysis. The fraction of microcolonies containing only a single cell, P_single (t), which indicates the frac

SUBMITTER: Islam MZ 

PROVIDER: S-EPMC10754687 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature